Figure 1.
Expression levels of MAF in CD34+ cells from MPN patients. Expression levels of MAF in CD34+ cells from MPN patients and HDs. Gene expression levels were measured by microarray analysis performed by Affymetrix platform as detailed in Norfo et al.8(please, see the ‘Materials and Methods’ section). MAF expression levels are reported as Robust Multiarray Analysis (RMA)-normalized log2 signals, which were obtained by using the Partek GS software. Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and bars show the range of values. Data in a are representative of 38 PMF, 27 ET, 24 PV and 30 HD samples (14 from BM and 16 from PB). Only PMF (n=38) and HD (n=30) samples are shown in b, where PMF samples are classified into JAK2-mutated (n=20), MPL-mutated (n=3), CALR-mutated (n=9) and triple-negative (n=6), based on the mutational status. Only JAK2V617F-positive PMF (n=20) and ET (n=16) samples are shown in c, where samples are classified based on the JAK2V617F allele burden. BM, bone marrow; ET, essential thrombocythemia; MPN, myeloproliferative neoplasm; PMF, primary myelofibrosis; PV, polycythemia vera; HD, healthy donor; PB, peripheral blood; n, number of samples.