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. 2017 Aug 8;32(2):438–449. doi: 10.1038/leu.2017.220

Figure 2.

Figure 2

Detection of a subset of secreted molecules in LMAFVAR1IDN-, LMAFVAR2IDN- and LXIDN-transduced cells and in PMF patients plasma by ELISA. Protein levels of seven secreted molecules (IL-8, a; CCL2, b; PLAUR, c; MMP9, d; LGALS3, e; SPP1, f; THBS1, g) were measured by ELISA in culture supernatants from LMAFVAR1IDN, LMAFVAR2IDN and LXIDN-transduced cells (subpanels i) and plasma from PMF patients and HDs (subpanels ii). Protein levels are expressed as ng/ml. Protein levels in culture supernatants from LMAFVAR1IDN-, LMAFVAR2IDN- and LXIDN-transduced cells (subpanels i) are reported as mean±standard error of the mean (s.e.m.). *P<0.05; **P<0.01 versus LXIDN. The results come from three independent experiments. Plasma levels in PMF patients and HDs (subpanels ii) are shown as dot plots. Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and the bars show the range of values. Data are representative of 30 PMF and 10 HD samples. Comparisons between HDs and PMF patients were performed by using the Mann–Whitney U test, and the chosen level of significance was P<0.05 with a two-tailed test. ELISA, enzyme-linked immunosorbent assay; HD, healthy donor; PMF, primary myelofibrosis.