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. 2017 Aug 8;32(2):438–449. doi: 10.1038/leu.2017.220

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Detection of SPP1 and LGALS3 in the plasma of MPN patients by ELISA. Protein levels of SPP1 (a) and LGALS3 (b) were measured by ELISA in plasma samples derived from PMF (n=115), ET (n=47) and PV (n=24) patients and HDs (n=60). Protein levels are expressed as ng/ml. Boxes represent the interquartile range that contains 50% of the subjects, the horizontal line in the box marks the median, and the bars show the range of values. Comparisons between two groups were performed by using the Mann–Whitney U test, and the chosen level of significance was P<0.05 with a two-tailed test. ELISA, enzyme-linked immunosorbent assay; ET, essential thrombocythemia; HDs, healthy donors; n, number of samples; PMF, primary myelofibrosis; PV, polycythemia vera.