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. 2018 Feb 6;8:22. doi: 10.3389/fcimb.2018.00022

Figure 3.

Figure 3

The essential role CagL plays in IL-8 and IL-6 induction in infected human endothelial cells is independent of CagA. (A) Mean secreted IL-8 levels in spent culture medium collected at 24 hpi from HUVECs inoculated with HI, or H. pylori strains P12 WT or various isogenic ΔcagL, ΔcagA, and ΔvirD4 single and double mutants at MOI = 10. Error bars denote SEM, N = 4; statistical analysis using two-way ANOVA (Tukey's multiple comparisons post-test); ****denotes conditions significantly different (p < 0.0001) to WT. (B) Mean secreted IL-6 levels in spent culture medium collected at 24 hpi from HUVECs inoculated with HI, or H. pylori strains P12 WT or various isogenic ΔcagL, ΔcagA, and ΔvirD4 mutants at MOI = 10. Error bars denote SD, N = 2; statistical analysis using two-way ANOVA (Tukey's multiple comparisons post-test); *, ***, and **** denote conditions significantly different (p < 0.05, p < 0.001, and p < 0.0001, respectively) to WT.