Figure 6.

Effect of Alox15 deficiency on cytochrome p450 2E1 (CYP2E1) expression and markers of liver inflammation. A: CYP2E1 protein expression analyzed by Western blotting with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control (Ctrl). B: The intensity of protein bands (from A) was quantified by densitometry using the ImageJ software. C: Tumor necrosis factor-α (TNF-α) mRNA. D: Macrophage inflammatory protein 2-α (MIP-2α) mRNA. C and D: Hepatic mRNA levels were measured by RT-PCR. Genes were normalized to 18S rRNA as an internal control. E: Representative images of chloroacetate esterase staining (CAE; arrows indicate CAE-positive neutrophils). F: Quantification of CAE staining performed by counting CAE-positive neutrophils in a random series of five digital images per animal. Results are presented as fold changes relative to the WT pair-fed group. Data are expressed as means ± SEM. Each experiment is a representative or the average of 5–8 mice per group. ^∗P < 0.05. Original magnification, ×400 (E). EtOH, ethanol; WT, wild-type.