Table 1.
Kinetic parameters determined from glutaminase assays as a function of temperature.
| Temp (K) | Activated | Basal | (kcat/Km)act/ (kcat/Km)basal | ||||
|---|---|---|---|---|---|---|---|
| kcat (s−1) | Km (mM) | kcat/Km | kcat(s−1) | Km (mM) | kcat /Km | ||
| 303.15 | 4.09 ± 0.13 | 1.30 ± 0.25 | 3.15 × 103 | 3.72 (0.04) × 10−3 | 4.91 ± 0.87 | 7.58 × 10−1 | 4,161 |
| 313.15 | 4.37 ± 0.12 | 1.35 ± 0.18 | 3.24 × 103 | 5.71 (0.04) × 10−2 | 4.71 ± 0.91 | 1.21 × 101 | 268 |
| 323.15 | 4.89 ± 0.11 | 1.64 ± 0.18 | 2.98 × 103 | 1.39 (0.01) × 10−1 | 3.80 ± 0.85 | 3.65 × 101 | 82 |
| 333.15 | 5.50 ± 0.15 | 1.36 ± 0.18 | 4.04 × 103 | 2.12 (0.01) × 10−1 | 3.89 ± 0.70 | 5.44 × 101 | 74 |
| 343.15 | 5.92 ± 0.18 | 1.32 ± 0.21 | 4.48 × 103 | 2.04 (0.08) × 10−1 | 2.96 ± 0.42 | 6.88 × 101 | 65 |
Michaelis–Menten parameters were determined in the presence (activated) and absence (basal) of saturating PRFAR.