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. 2018 Feb 6;9:51. doi: 10.3389/fphar.2018.00051

Figure 5.

Figure 5

Effect of pistachios on adhesion molecules expression. Immunohistochemical analysis of myocardial sections obtained from STZ rats subjected to myocardial ischemia/reperfusion revealed a positive staining for ICAM-1 (C) and P-selectin (D) in the injured tissues, which was primarily localized around the vessel. There was no detectable immunostaining for ICAM-1 (E) and P-selectin (F) in the heart tissues obtained from rats treated with NP. Treatments with RP did not provide a significant reduction in ICAM-1 (G) and P-selectin (H) tissue expression. No positive staining was found in tissues collected from sham treated rats (A,B). Densitometry analysis of immunocytochemistry photographs (n = 5 photographs from all samples collected from each rats in different groups) for ICAM-1 (I) and P-selectin (L) was assessed. The assay was carried out by Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Data are indicated as % of total tissue area. This figure is expressive of at least 3 experiments executed on different experimental days. Data are means ± S.E.M. of 10 rats for each group. *P < 0.05 vs. sham, °P < 0.05 vs. ischemia-reperfusion.