Figure 3.

Quantitation using linearized versus circular pBRAF-HET calibrator plasmid. A: Mutant allele burden for 14 HCL samples was calculated using linear and circular pBRAF-HET calibrator (pHET method) and plotted against data from the same samples calculated with a standard curve–based calculation of mutant allele burden (STD method). Averages from three experiments are shown. B: Schematic of the circular and linear pBRAF-HET plasmid.