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Immunocytochemistry analysis of ATP2B1-4 expression in mesencephalic dopaminergic neurons. (A) Overview protocol for making mesDA in vitro for 45 days. (B) Immunostaining of mesDA with double positive of FOXA2 and Tyrosine Hydroxylase (TH) markers indicates the efficient protocol. (C) Immunostaining of ATP2B1-4 and TH expression. Arrow heads point to the TH positive staining with ATP2B1-4 staining and white lines in ATP2B1-4 panels outlines TH positive staining. Scale bars = 20 μm.
