Table 1.
MRM scan parameters for PteGlu, H4folate, 5-CH3-H4folate, 5-CHO-H4folate, and the respective internal standards.
| Compound | Precursor [m/z] | Product [m/z] | Dwell time [ms] | Q1 Pre bias [V] | CE [V] | Q3 Pre bias [V] |
|---|---|---|---|---|---|---|
| PteGlu | 442.30 | 295.15 | 50.0 | −13.0 | −16.0 | −16.0 |
| 176.20 | 50.0 | −13.0 | −37.0 | −20.0 | ||
| 120.05 | 50.0 | −13.0 | −35.0 | −14.0 | ||
| [13C]-PteGlu | 447.2 | 295.15 | 50.0 | −13.0 | −16.0 | −16.0 |
| 176.20 | 50.0 | −13.0 | −37.0 | −20.0 | ||
| 120.05 | 50.0 | −13.0 | −35.0 | −14.0 | ||
| H4Folat | 446.0 | 299.20 | 50.0 | −22.0 | −20.0 | −16.0 |
| 120.10 | 50.0 | −22.0 | −37.0 | −14.0 | ||
| 166.15 | 50.0 | −22.0 | −41.0 | −19.0 | ||
| [13C]-H4Folat | 451.3 | 299.20 | 50.0 | −22.0 | −20.0 | −16.0 |
| 120.10 | 50.0 | −22.0 | −37.0 | −14.0 | ||
| 166.15 | 50.0 | −22.0 | −41.0 | −19.0 | ||
| 5-CH3-H4Folat | 460.2 | 313.20 | 50.0 | −13.0 | −20.0 | −17.0 |
| 180.15 | 50.0 | −13.0 | −37.0 | −14.0 | ||
| 194.25 | 50.0 | −23.0 | −33.0 | −22.0 | ||
| [13C]-5-CH3-H4Folat | 465.3 | 313.2 | 50.0 | −13.0 | −20.0 | −17.0 |
| 180.15 | 50.0 | −13.0 | −37.0 | −14.0 | ||
| 194.25 | 50.0 | −23.0 | −33.0 | −22.0 | ||
| 5-CHO-H4Folat | 474.3 | 327.15 | 50.0 | −14.0 | −20.0 | −17.0 |
| 299.20 | 50.0 | −14.0 | −31.0 | −16.0 | ||
| 208.20 | 50.0 | −18.0 | −36.0 | −24.0 | ||
| [13C]-5-CHO-H4Folat | 479.25 | 327.15 | 50.0 | −14.0 | −20.0 | −17.0 |
| 299.20 | 50.0 | −14.0 | −31.0 | −16.0 | ||
| 208.20 | 50.0 | −18.0 | −36.0 | −24.0 |