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. 2017 Sep;187(9):1984–1997. doi: 10.1016/j.ajpath.2017.05.009

Figure 6.

Figure 6

Pleural mesothelial stomata and exfoliation in ADN–VEGF-C mice. A and B: Scanning electron microscopic (SEM) images of pleural mesothelium of control mouse (A) and ADN–VEGF-C mouse on 0.075 mg/mL doxycycline for 7 days (B). A: Left panel: Cuboidal mesothelial surface of parietal pleura. Middle panel: Boxed area from left panel shown at higher magnification, indicating two lymphatic stomata (arrows). Right panel: Flat mesothelial surface of parietal pleura. No stomata are present in this region. B: ADN–VEGF-C mouse. Left panel: Surface of parietal pleura showing large region lacking mesothelial cells but revealing foramina in matrix (arrowheads). Right panel: Boxed area from left panel shown at higher magnification, indicating abnormal mesothelial cells surrounded by region of exfoliation. C: Amount of mesothelial exfoliation and corresponding volume of chyle in the thorax of ADN–VEGF-C mice on doxycycline for 0 to 7 days. D: Drawings of exfoliated regions in three ADN–VEGF-C mice. Green marks regions of exfoliation in only one of the three mice; yellow, regions of colocalization of exfoliation in two of the three mice; and red, colocalization in all three mice. E: Drawings of the location of lymphatic plexuses in three Prox1-GFP control mice, as in D. F: Comparison of amount of mesothelial exfoliation 24 hours after intrathoracic injection of phosphate-buffered saline (PBS) or chyle in wild-type mice. SEM image of exfoliated region after intrathoracic injection of chyle into a wild-type mouse (arrows). n = 2 mice per group (C, chyle volume on day 7); n = 3 or 4 mice per group (B and C, other groups); n = 3 mice per group (F). ∗P < 0.05 versus values for days 0, 1, 2, and 3 and for chyle volumes on days 0, 1, and 2 (one-way analysis of variance). Scale bars: 60 μm (A, left and right panels, B, left panel, and F); 5 μm (A, middle panel, and B, right panel).