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. 2018 Feb 6;12:44. doi: 10.3389/fnins.2018.00044

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Copyright © 2018 Kimura, Sharma, Ishiguro and Hisanaga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphoisotypes of tau on Phos-tag SDS-PAGE. (A) Immunoblotting of tau expressed in COS-7 cells with tau5 in the presence (+) or absence (−) of Cdk5-p35 after Phos-tag-SDS-PAGE, and the phosphorylation sites in each band are indicated by the amino acid number according to the human longest isoform of tau. Y is an unknown phosphorylation site. (B) Immunoblotting of tau in human brains after Phos-tag SDS-PAGE. NC, normal control; AD V and VI, Braak stage V and VI of Alzheimer's disease (AD); CBD Tl and Pg, temporal lobe (Tl) and prefrontal gyrus (Pg) of corticobasal degeneration (CBD). We propose that the banding pattern created using Phos-tag SDS-PAGE could be read by a bar code reader and result in a simple method for diagnosing tauopathy. (A,B) are reproduced and modified from figures reported previously by Kimura et al. (2016a,b), respectively.