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. 2018 Feb 6;9:65. doi: 10.3389/fphys.2018.00065

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Copyright © 2018 Sandoval, Lazcano, Ferrari, Pinto-Pardo, González-Billault and Utreras.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TNF-α increases ROS production in primary culture of mouse TG and DRG neurons. Representative images of primary cultures loaded with DCFH-DA (DCF) and CMTMR (CT) probes from TG (A) and DRG (B) neurons treated with TNF-α alone or with roscovitine or VAS2870. (C,D) Quantification of fluorescence intensity of DCF normalized by CT under each corresponding treatment in TG neurons (C) or DRG neurons (D). Scale bars are 20 μm. The bar graphs represent mean ± SD of n = 3 different experiments. Statistical differences correspond to a one-way ANOVA with a a Bonferroni's multiple comparison test. Number of analyzed neurons for each treatment is inside the corresponding bar.