Figure 4.

MID Clustering-based IR-Seq is sensitive to detect both low copy and highly clonal expanded T cell receptors (TCRs). (A) Number of RNA molecules detected by sequencing for each spike-in TCR control sequences (the numbers in the legend denote copies of each TCR spike-in control sequence added). (B) Comparison of clone size distribution in naïve CD8⁺ T cells and CMVpp65-specific effector CD8⁺ T cells (dashed line indicates TCR sequences with 20 copies of RNA molecules). (C) The percentage of RNA molecules that varying degree of clonally expanded complementarity-determining region 3 account for.