Table 1.
Spike-in Jurkat T cell receptor (TCR) RNA detection in naïve CD8+ T cells.
| Sample | Jurkat TCR copies detected |
|---|---|
| 20,000Tn_10%RNA | 7 |
| 20,000Tn_30%RNA | 0 |
| 20,000Tn_50%RNA | 1 |
| 100,000Tn_10%RNA | 5 |
| 100,000Tn_30%RNA | 4 |
| 100,000Tn_50%RNA | 1 |
| 200,000Tn_10%RNA | 7 |
| 200,000Tn_30%RNA | 3 |
| 200,000Tn_50%RNA | 3 |
| 1,000,000Tn_10%RNA | 4 |
| 1,000,000Tn_30%RNA | 8 |
| 1,000,000Tn_50%RNA | 17 |
10 TCR-copy worth of Jurkat RNA was added to each sample during the reverse transcription step. Number of molecular identifiers for RNA molecules that are tagged with jurkat TCR sequences were counted.