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Tachyphylaxis of 25CN-NBOH (0.75 or 1.5 mg/kg, s.c.) induced head twitches (HTRs) (as observed for the first 30 min post-application). Agonist was applied twice at an interval of 60 and 90 min, respectively. Mean + SEM; n = 5–6 per group. Repeated measures ANOVA, Bonferroni-corrected post-hoc comparison to respective first application (0 min), ##p ≤ 01.
