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. 2018 Feb 6;12:29. doi: 10.3389/fncel.2018.00029

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Copyright © 2018 Moy, Khoutorsky, Asiedu, Dussor and Price.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BDNF protein is increased in WT but not eIF4E^S209A DRGs after PAR2 activation. (A) Western blot analysis shows an increase in BDNF protein standardized to GAPDH in WT DRGs (L4-L6) ipsilateral (IPSI) to 2at-LIGRL intraplantar (i.pl.) injection compared to contralateral (CON) (n ≥ 5, t = 3.662, df = 9, ^∗∗p = 0.0052, t-test). Protein and mRNA extraction was done 24 h following i.pl. injection. (B) eIF4E^S209A DRG (L4-L6) showed no differences in BDNF protein levels between IPSI and CON (n = 6, p > 0.05, t-test). mRNA levels of Bdnf-pan and the Bdnf-201 isoform were analyzed using qPCR. (C,D) While Bdnf-pan mRNA levels after 2at-LIGRL injection were not changed in DRGs from both genotypes (n ≥ 3, t-test). (E,F) Bdnf-201 mRNA levels were significantly increased in both WT and eIF4E^S209A DRGs (WT: n ≥ 3, t = 3.055, df = 5, ^∗p = 0.0283, t-test; eIF4E^S209A: n ≥ 3, t = 2.756, df = 5, ^∗p = 0.04, t-test).