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. 2018 Feb 7;9:199. doi: 10.3389/fimmu.2018.00199

Figure 3.

Figure 3

CD137+CD154− expression identifies stable regulatory T cells (Tregs) ex vivo. (A) CD137 and CD154 expression were analyzed on CD25+CD127− Tregs after 6-h stimulation with anti-CD3/anti-CD28 ex vivo (n = 68, 17 independent experiments were performed). (B) Frequencies of FoxP3+ cells were analyzed among CD137- and CD154-expressing cells within the CD25+CD127− Treg compartment (n = 30, five independent experiments were performed). (C) CD137+CD154− and CD137+CD154+ Tregs (CD25+CD127−CD45RO+) were sorted after 6-h stimulation with anti-CD3/anti-CD28, and methylation of indicated regions was analyzed (data from two independent experiments are shown, and five and six male donors were pooled for each experiment). Methylation of central memory and effector memory T cells was derived from the study by Durek et al. (55). (A,B) Each dot represents one donor, lines indicate (A,B) median, (C) mean ± SEM is shown; statistical significances were determined by (B) Kruskal–Wallis test.