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. 2018 Feb 7;9:199. doi: 10.3389/fimmu.2018.00199

Figure 6.

Figure 6

Phenotype of antigen-activated chimeric antigen receptor (CAR)–regulatory T cells (Tregs). (A–H) LNGFR+ CAR–Tregs were sorted and expanded for 10–12 days before analysis of (A) LNGFR expression and (B) dextran-binding (n = 12 for LNGFR+ sorted and n = 11 for LNGFR− sorted from four independent experiments). (C,D) FoxP3 expression in LNGFR+ sorted CAR–Tregs was analyzed ex vivo and after 21 days; (D) representative dot plot of one donor and (C) statistical summary of several donors (n = 6 from two independent experiments). (E–H) CAR–Tregs were restimulated for 6 h with bead-bound dextran and (E,G) FoxP3 expression (n = 6 from two independent experiments) and (E,F) CD137 and CD154 expression (n = 12 for from four independent experiments) were analyzed; (E) representative dot plot of one donor and (F,G) statistical summary of several donors. (H) Cytokine expression was analyzed on CD137- and CD154-expressing CAR–Tregs (n = 6 from two independent experiments). (A–C,F–H) Each dot represents one donor, and statistical significances were determined by (C) paired t-test or (H) Wilcoxon signed-rank test; lines indicate (A,B) median or (F–H) mean.