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. 2018 Feb 7;9:63. doi: 10.3389/fphar.2018.00063

FIGURE 2.

FIGURE 2

Expression of SNAT2V5 in HeLa cells. (A) HeLa cells were transiently transfected with 5 or 10 μg pcDNA6 either lacking (empty vector, EV) or containing SNAT2V5; 48 h post-transfection cells were lysed and whole cell lysates immunoblotted with antibodies to V5, SNAT2, and actin. (B) HeLa cells transiently expressing SNAT2V5 were incubated in the absence or presence of 1× physiological AA mix for 8 h and then subsequently lysed and immunoblotted using antibodies to V5 or the α1-subunit of the Na,K-ATPase (loading control). (C) Cells transfected with pcDNA6V5 (EV) or pcDNA6 containing SNAT2V5 were incubated in the absence or presence of 1× physiological AA mix for 8 h prior assaying Me-AIB uptake. Uptake values are mean ± SEM from three separate experiments, each conducted in triplicate. Asterisks indicate a significant difference between the indicated bars P ≤ 0.05.