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. 2018 Feb 7;9:172. doi: 10.3389/fimmu.2018.00172

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Copyright © 2018 Hou, Zhou, Yu, Mao, Bosco, Wang, Lu, Mao, Wu, Wang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reproducibility of intracellular IFN-γ detection by flow cytometry. Five healthy donors were recruited and three samples were taken from each individual (the time interval between two samples was 1 week). Intracellular staining of IFN-γ was performed in diluted whole blood after stimulated with Leukocyte Activation Cocktail (BD GolgiPlug™) for 4 h. The percentages of IFN-γ⁺ cells in CD4⁺, CD8⁺ T cells, and NK cells of each individual are shown in bar graphs. Data are shown as mean ± SD. CV, coefficient of variation.