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. 2018 Feb 7;9:172. doi: 10.3389/fimmu.2018.00172

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Copyright © 2018 Hou, Zhou, Yu, Mao, Bosco, Wang, Lu, Mao, Wu, Wang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the reference intervals (RIs) of lymphocyte function in another center. Diluted whole blood from another 100 healthy adults recruited from another center was stimulated with Leukocyte Activation Cocktail (BD GolgiPlug™) for 4 h. The production of IFN-γ in CD4⁺, CD8⁺ T cells, and NK cells was measured by flow cytometry. Scatter plots showing the percentages of IFN-γ⁺ cells in CD4⁺, CD8⁺ T cells, and NK cells. The upper and lower dashed lines showing our established RIs of lymphocyte function.