Figure 3.

Alternative splicing of ncRNA LOC9270896 skipped exons and retained introns in milk seed, embryo, and endosperm. (A) Gene structure. Primers flanking skipped exons and retained introns are indicated by arrows. (B) RT-PCR analysis of retained intron amplicons (MS, milk seed; emb, embryo; endo, endosperm). The 1,455 bp band containing all three introns is present in 15 of 20 predicted variants, whereas the 593 bp band from which all introns are spliced out is not predicted but predominates in embryo. (C) Exon skipping is seen in milk seed. In embryo exon retention and an unannotated differential 5′ splice site variant are present.