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. 2018 Feb 7;9:62. doi: 10.3389/fphys.2018.00062

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Copyright © 2018 Liu, Zhu, Dai, Pan, Li, Wang, Yang, Yan and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of ZAG on the expression of WAT browning-related genes in iWAT in HFD-induced obese mice. (A) Immunostaining for UCP1 in mouse iWAT. (B) The mRNA levels of Zag, Pgc1a, and Ucp1 in mouse iWAT. (C) Correlation of mRNA levels of Zag and Pgc1a in mouse iWAT. Spearman's rank correlation analysis was applied to determine the correlation. (D,E) The protein levels of UCP1 (D) and PGC1a (E) in mouse iWAT. The relative integrated density of each protein band was quantified using the program ImageJ, with β-actin as the internal control. Three representative protein band figures are shown. Data are presented as the mean ± s.e.m. SF n = 10, HFD n = 13, ZAG n = 8. iWAT, inguinal white adipose tissue. ZAG, zinc-α2-glycoprotein; UCP1, uncoupling protein 1; PGC1α, peroxisome proliferator-activated receptor gamma coactivator 1 alpha. ^*P < 0.05 vs. HFD.