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. 2018 Feb 7;9:62. doi: 10.3389/fphys.2018.00062

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Copyright © 2018 Liu, Zhu, Dai, Pan, Li, Wang, Yang, Yan and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of ZAG on the expression of WAT browning-related genes in pVAT and BAT in HFD-induced obese mice. (A) The protein levels of ZAG in mouse pVAT. (B) The mRNA levels of Pgc1a and Ucp1 in mouse pVAT. (C) The protein levels of PGC1α in mouse pVAT. (D) The mRNA levels of Pgc1a and Ucp1 in mouse BAT. For the western blots shown in (A) and (C), the relative integrated density of each protein band was quantified using the program ImageJ with GAPDH as the internal control. Three representative protein band figures were shown. Data are presented as the mean ± s.e.m. SF n = 10, HFD n = 13, ZAG n = 8. pVAT, perirenal visceral adipose tissue; BAT, brown adipose tissue; ZAG, zinc-α2-glycoprotein; UCP1, uncoupling protein 1; PGC1α, peroxisome proliferator-activated receptor gamma coactivator 1 alpha. ^#P < 0.05 vs. SF; ^*P < 0.05 vs. HFD.