Skip to main content
. 2017 Dec 15;61(3):1204–1217. doi: 10.1021/acs.jmedchem.7b01674

Figure 5.

Figure 5

Cellular inhibitory activity of compounds 29, 30, and 36. HEK293 cells were cotransfected with FLAG tagged PRMT3 (wt) or its catalytic mutant (mt) and GFP-tagged histone H4 and treated with different concentrations of compounds, as indicated. Total cell lysates were collected 24 h post inhibitor treatment and analyzed for H4R3me2a, GFP, and FLAG levels by Western blotting. The graphs represent nonlinear fits of H4R3me2a fluorescence intensities normalized to GFP. The results are the averages of three replicates.