FIG. 2.

Impact of enrichment for viable leukemic blasts on RNA quality. Figure shows the positive impact of enrichment on the global quality of RNA. Specimens in the lowest and highest tertiles of viability by DAPI are included. Figure shows the correlation between viability (DAPI, x-axis) and RIN (y-axis) in unsorted MNCs (A) and enriched blasts (B) from bone marrow (light gray circles) and peripheral blood (dark gray squares). The majority of specimens with poor viability and low RIN numbers (i.e., suboptimal RNA quality) in unsorted MNCs demonstrated an improvement in RIN numbers after enrichment for viable AML blasts (RIN <6, suboptimal RNA quality, lower black line; RIN >8, higher quality RNA, upper black line). RIN, RNA integrity number.