Table 1.
Multivariable Mixed Model of Gene Expression Fold Changes
| Sample source | Subpopulation | Immunophenotype | FLT3 mutation | NPM1 mutation | Cytogenetics | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PB vs. BM | Blast vs. MNC | CD117 and other vs. CD34 | Pos vs. neg | Pos vs. neg | Oth, NS, and Rej vs. NN | ||||||||||
| Gene | Coef. | p | Coef. | p | 1 vs. 0 Coef. | 2 vs. 0 Coef. | p | Coef. | p | Coef. | p | Oth vs. NN Coef. | NS vs. NN Coef. | Rej vs. NN Corr. | p |
| BAALC | 1.10 | 0.48 | 1.57 | <0.001 | 0.25 | 0.07 | <0.001 | 1.37 | 0.27 | 0.21 | <0.001 | 1.39 | 0.98 | 0.98 | 0.67 |
| CCNA1 | 0.72 | 0.003 | 1.14 | 0.0742 | 0.75 | 0.37 | 0.06 | 2.11 | 0.03 | 5.00 | <0.001 | 0.48 | 1.21 | 0.99 | 0.14 |
| CD34 | 1.07 | 0.71 | 1.16 | 0.2864 | 0.02 | 0.01 | <0.001 | 1.49 | 0.27 | 0.23 | 0.001 | 1.09 | 1.43 | 2.52 | 0.24 |
| CEBPA | 0.93 | 0.29 | 0.89 | 0.0190 | 0.84 | 0.63 | 0.14 | 1.12 | 0.54 | 1.91 | 0.003 | 1.01 | 1.43 | 1.49 | 0.25 |
| ERG1 | 0.87 | 0.41 | 1.10 | 0.4464 | 0.33 | 0.09 | <0.001 | 2.01 | 0.01 | 0.92 | 0.80 | 1.36 | 1.05 | 1.39 | 0.65 |
| EVI1 | 0.27 | 0.05 | 1.61 | 0.2452 | 0.88 | 0.53 | 0.94 | 2.02 | 0.63 | 0.12 | 0.21 | 5.8 | 0.52 | 0.10 | 0.23 |
| FLT3 | 0.78 | 0.001 | 1.11 | 0.0347 | 0.77 | 0.59 | 0.05 | 1.47 | 0.03 | 1.62 | 0.02 | 1.25 | 1.21 | 1.32 | 0.53 |
| GATA2 | 1.22 | 0.13 | 0.90 | 0.2585 | 0.48 | 0.18 | <0.001 | 2.23 | 0.003 | 2.85 | 0.001 | 1.3 | 1.98 | 2.07 | 0.11 |
| IL3RA | 1.22 | 0.09 | 0.91 | 0.2902 | 0.83 | 0.41 | 0.001 | 1.83 | 0.002 | 1.47 | 0.09 | 1.04 | 1.15 | 1.06 | 0.97 |
| JAG1 | 0.60 | 0.01 | 0.46 | <0.001 | 1.84 | 1.71 | 0.55 | 1.53 | 0.36 | 7.88 | <0.001 | 2.77 | 2.46 | 3.07 | 0.13 |
| KIT | 0.80 | 0.07 | 1.01 | 0.8941 | 0.51 | 0.11 | <0.001 | 1.34 | 0.21 | 1.02 | 0.95 | 1.68 | 1.48 | 2.12 | 0.05 |
| MN1 | 1.22 | 0.30 | 1.04 | 0.7599 | 0.17 | 0.04 | <0.001 | 1.24 | 0.55 | 0.34 | 0.01 | 1.2 | 1.58 | 1.76 | 0.60 |
| RUNX1 | 0.90 | 0.37 | 0.99 | 0.9097 | 0.47 | 0.41 | <0.001 | 1.35 | 0.08 | 1.62 | 0.02 | 1.27 | 1.45 | 1.60 | 0.14 |
| WT1 | 0.95 | 0.82 | 1.15 | 0.3540 | 0.11 | 0.03 | <0.001 | 6.20 | 0.001 | 13.49 | <0.001 | 2.23 | 8.90 | 3.89 | 0.04 |
Results of multivariate mixed-model analyses fit for each gene, allowing the mean log expression levels to vary with the following factors: sample source (PB vs. BM), cellular subpopulation (Blasts vs. MNCs), cytogenetics (normal [NN], other [Oth, any abnormal clone], rejected [Rej], not submitted [NS]), IP group (coded in this table as 0 = CD34+, 1 = CD34−CD117+, and 2 = Other, CD34−CD117−), FLT3-ITD, and NPM1 mutation status (positive [Pos] vs. negative [Neg]) while allowing for correlation of expression between sample sources and cellular subpopulations from the same patient. Entries in the table are average fold changes between categories of each factor (coef. = coefficient).
BM, bone marrow; MNCs, mononuclear cells; PB, peripheral blood.