REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-β-Actin	Sigma-Aldrich	Cat#A5441; RRID: AB_476744
Rat monoclonal anti-CD8a	BioXCell	Cat#BE0061; RRID: AB_1125541
Rat monoclonal anti-CD16/CD32	BD Biosciences	Cat#553141; RRID: AB_394656
BV510 Rat monoclonal anti-CD45	BD Biosciences	Cat#563891
Rat monoclonal anti-CD3	BD Biosciences	Cat#560527; RRID: AB_1727463
FITC Rat monoclonal anti-CD8a	Biolegend	Cat#100706; RRID: AB_312745
Brilliant Violet Rat monoclonal anti-F4/80	Biolegend	Cat#123137; RRID: AB_2563102
APC Rat monoclonal anti-CD11b	Biolegend	Cat#101212; RRID: AB_312795
APC Rat IgG2b, kappa Isotype Ctrl antibody	Biolegend	Cat#400611; RRID: AB_326555
Rat monoclonal anti-IFNγ	BD Biosciences	Cat#562018; RRID: AB_10896992
Rat anti-PD-1 IgG2a (RMP1-14)	BioXCell	Cat#BE0146; RRID: AB_10949053
Rat anti-IgG2a Isotype Control Clone 2A3	BioXCell	Cat#BE0089; RRID: AB_1107769
Rat monoclonal anti-CD8a	eBioscience	Cat#14-0808; RRID: AB_2572860
Rat monoclonal anti-F4/80	Serotec	Cat#MCAP497; RRID: AB_2335598
Rabbit monoclonal anti-Ki67	Cell signaling	Cat#12202; RRID: AB_2620142
Rabbit monoclonal anti-c-MYC	Cell signaling	Cat#13987; RRID: AB_2631168
Rabbit monoclonal anti-Acetyl α Tubulin	Cell signaling	Cat# 5335; RRID: AB_10544694
Rabbit monoclonal anti-Acetylated Histone 3	Cell signaling	Cat#4499; RRID: AB_10544537
Rabbit polyclonal anti-Histone H3	Cell signaling	Cat#9715; RRID: AB_331563
Rabbit polyclonal anti-DNMT1	Sigma Aldrich	Cat#D4692; RRID: AB_262096
Anti-rabbit secondary (western blot)	GE Healthcare	Cat#NA934V
Anti-mouse secondary (western blot)	GE Healthcare	Cat#NA931V
Mouse monoclona IgG1 Isotype Control	Cell Signaling	Cat#5415; RRID: AB_10829607
Rabbit anti-rat Biotinylated IgG	Vector	Cat#BA-4001; RRID: AB_10015300
Bacterial and Virus Strains
Ad-CMV-Cre	Viral Vector Core, University of Iowa	Cat#VVC-U-IOWA-5
Biological Samples
Patient-derived xenografts (PDX)	The Jackson Laboratory	Cat#TM00302
Chemicals, Peptides, and Recombinant Proteins
Azacidine	Sigma-Aldrich	Cat#A2385
ITF-2357	Apexbio	Cat#A4093
MS-275	Syndax	MTA
MCGD0103	Apexbio	Cat#A4089
RGFP996	Apexbio	Cat#A8803
Tubastatin	Apexbio	Cat#A4101
Vorinostat	Cell signaling	Cat#12520
Puromycin	Sigma-Aldrich	Cat#P7255
DMSO	Sigma	Cat#D2660
RPMI1640	Corning	Cat#10-040
Fetal Bovine Serum	Hyclone	Cat#SH30910.03
0.25% Trypsin	Corning	Cat#25-053
DMEM	Corning	Cat#10-013
Pen/Strep	GIBCO	Cat#15140-122
m-CSF murine	Peprotech	Cat#315-02-100UG
ECM gel	Sigma Aldrich	Cat#E2170-10ML
Lipofectamine 2000	Invitrogen	Cat#11668-022
Fixable Viability Dye	eBioscience	Cat# 65-0865-14
eBiosciences Stim cocktail	eBioscience	Cat# 00-4970-03
Propidium Iodide	eBioscience	Cat#00-6990-50
Critical Commercial Assays
RNeasy Mini kit	QIAGEN	Cat#74104
RNeasy Microkit	QIAGEN	Cat#74004
RNase free DNase Set	QIAGEN	Cat#79254
qScript cDNA SuperMix	Quanta	Cat#95048-025
iTaq universal sybr green supermix	BioRad	Cat#1725124
Homogenizer mini column	Omega	Cat#HCR003
Purelink midiprep	Invitrogen	Cat#K210014
Cell titer AQueous one	Promega	Cat#G3582
BrdU Cell Proliferation ELISA	Calbiochem	Cat#QIA58
Mouse Cytokine Array Q1	Raybiotech	Cat#QAM-CYT-1-1
Sigma Whole Transcriptome Amplification kit	Sigma Aldrich	Cat#WTA2-10RXN
Deposited Data
Raw and analyzed data for gene expression	This paper	GEO: GSE104244
Experimental Models: Cell Lines
NCI-H1755 (	ATCC	RRID: CVCL_1492
NCI-H520	ATCC	RRID: CVCL_1566
NCI-H1650	ATCC	RRID: CVCL_1483
NCI-H1975	ATCC	RRID: CVCL_1511
NCI-H441	ATCC	RRID: CVCL_1561
NCI-H661	ATCC	RRID: CVCL_1577
NCI-H596	ATCC	RRID: CVCL_1571
HCC4006	ATCC	RRID: CVCL_1269
NCI-H1703	ATCC	RRID: CVCL_1490
NCI-H838	ATCC	RRID: CVCL_1594
NCI-H2170	ATCC	RRID: CVCL_1535
NCI-H1792	ATCC	RRID: CVCL_1495
NCI-H460	ATCC	RRID: CVCL_0459
NCI-H23	ATCC	RRID: CVCL_1547
A-549	ATCC	RRID: CVCL_0023
NCI-H1299	ATCC	RRID: CVCL_0060
HCC827	ATCC	RRID: CVCL_DH92
LLC1	ATCC	RRID: CVCL_4358
Experimental Models: Organisms/Strains
Mouse: B6.129S4-Krastm4Tyj/J	The Jackson Laboratory	RRID: IMSR_JAX:008179
Oligonucleotides
shMYC (TRCN0000010391)	RNAi Consortium	Cat# TRCN0000010391
Primers for qRT-PCR, see Table S1	This paper	N/A
shDNMT1
MYC vector	(Cheng et al., 2013)	Addgene plasmid # 46970
pLV-AcGFP-N1	Clontech	Cat#632154
Recombinant DNA
Syncytin-1 (5′ LTR-Syn-1-pGL3)	(Chiappinelli et al., 2015)	N/A
ERV-3 (p3Xflag-CMV-14)	(Chiappinelli et al., 2015)	N/A
pE-GFP-N1	Clontech Laboratories	Cat#632469
pMD2.G		Addgene # 12259
psPAX2	Addgene	Addgene plasmid #12260
Software and Algorithms
GraphPad Prism	GraphPad Software	https://www.graphpad.com. RRID: SCR_002798
Gene set enrichment Analysis	(Subramanian et al., 2005)	http://www.broadinstitute.org/gsea. RRID: SCR_003199