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. 2018 Feb 12;17:21. doi: 10.1186/s12934-018-0872-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Simultaneously deletion of two non-continuous large-scale genome regions in L. casei BL23. a Layout and genetic context of the ~ 12.8 kb genome region and the sizes of PCR products amplified with primers H1F and H2R from BLD3 strains. b Colony PCR results. M: DNA marker; 1–2: random picked chloramphenicol resistance colonies. c Growth of L. casei BL23 and the deletion strain L. casei BLD3 on MRS