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. 2018 Feb 12;17:21. doi: 10.1186/s12934-018-0872-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Sequential deletion of double large-scale genome regions in L. casei BL23. a Genomic deletions in the double-deletion mutants of L. casei BL23. The red boxes represent the 1.68% deleted regions. b Colony PCR results. M: DNA marker; C1: L. casei BL23 (12.8 kb); C2: L. casei BL23 (39.3 kb); 1: random picked chloramphenicol resistance colonies (12.8 kb); 2: random picked chloramphenicol resistance colonies (39.3 kb)