Table 3.

Oligonucleotide primers used in this study

Primer	Sequence (5′–3′)a	Restriction site
H1F	GAAGAAGATCATTTACTCGAG	XhoI
H1R	TACCGTTCGTATAGCATACATTATACGAAGTTATCAGAACGCAAATCTCTTCTA
H2F	TGTATGCTATACGAACGGTAGTCGACTTACTTAATGCTATTCATTA	SalI
H2R	TTAAGATCTACATCGAGTTCAGCAAGCTAA	BglII
H3F	ATATGTCGACATTACAGTTACAAGCCATATAC	SalI
H3R	TCAATATTCTTCTCCGTCCC
H4F	GGGACGGAGAAGAATATTGAAGATCTCACTGCAGTATATCAGGTAACAAAAAGT	BglII, SalI
H4R	ACATGCATGCTTCCCTCTCCAAATATGCAC	SphI
H5F	CGGGATCCAATAGCTCAGATTTTTAACAACA	BamHI
H5R	TACCGTTCGTATAGCATACATTATACGAAGTTATTAATTTATCAAAAACCTTATTC
H6R	CCCAAGCTTGTACATCATACTGTTCATGCC	HindIII
H6F	TGTATGCTATACGAACGGTACTCGAGAAGCCATCGCTGCTAACAAA	XhoI
H7F	TTTTGGCCAAGCTGGTTTT
H7R	TTGTTTTTTCGCGGTACTGA
H8F	TCAGTACCGCGAAAAAACAAGATCTCACACTCGAGTGTAGTCGATAAAAATT	BglII, XhoI
H8R	GCA ACATGCATGCAGACCATGAGACTATTTCAG	SphI
PrF	TATAGCATACATTATACGAAGTTATCCAGCTAGGCCTAGTGTCCGT
PrR	CGTCAATACCTCCTAATTGA
galEF	TCAATTAGGAGGTATTGACGATGACAATTGCAGTTTTAGG
galER	TTACTCGAGTCAATTCCGGTCACCAAATC	XhoI
catF1	CCGCTCGAGATAACTTCGTATAATGTATGCTATA	XhoI
catR1	CCGCTCGAGATAACTTCGTATAGCATACATTATA	XhoI
catF2	CCGCTCGAGATAACTTCGTATAGCATACATTATACGAACGGTACGAAAGTCGACGGCAATAG	XhoI
catR2	GGAAGATCTCTGTAATATAAAAACCTTCTTC	BglII

^aThe restriction sites in the primer sequences are underlined