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. Author manuscript; available in PMC: 2018 Nov 20.
Published in final edited form as: Neurosci Lett. 2017 Oct 2;661:137–142. doi: 10.1016/j.neulet.2017.09.063

Table 1.

Treatment groups.

Cohort 1
Group Number Treatment Number of animals per group
1 Facility rearing only (the Control group) 5
2 Anesthesia with sevoflurane for 60 min at P6 (the Sevoflurane group) 6
3 Bumetanide prior to anesthesia with sevoflurane for 60 min at P6 (the Bumetanide plus Sevoflurane group) 5
Cohort 2
Group Number Treatment Number of animals per group
1 Facility rearing only (the Control group) 12
2 Anesthesia with sevoflurane for 60 min at P6 (the Sevoflurane group) 12
3 Maternal separation for 180 min at P10 (the MS10 group) 12
4 Anesthesia with sevoflurane for 60 min at P6 plus maternal separation for 180 min at P10 (the Sevoflurane plus MS10 group) 12
5 Bumetanide prior to sevoflurane at P6 plus maternal separation for 180 min at P10 (the Bumetanide plus Sevoflurane plus MS10 group) 12