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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Vet Parasitol. 2017 Dec 20;251:27–33. doi: 10.1016/j.vetpar.2017.12.015

Fig. 2.

Fig. 2

Alterations in host gene expression profiles in cells following C. parvum infection and overexpressing Cdg2_FLc_0220. (A) Genome-wide transcriptome analysis in INT cells following C. parvum infection and cells after transfection of the Full-Cdg2_FLc_0220 for 48 h, taking the fold changes >1.3 combined with a p < 0.05 as the threshold. The total numbers of genes whose expression is significantly altered after infection or transfection are shown. (B) Heatmaps representing the overlapping genes either upregulated or downregulated in cells following Full-Cdg2_FLc_0220 transfection and C. parvum infection. Expression levels of RNAs were presented as the fold changes normalized to the mean value of the non-infected control. (C) Downregulation of the DZLP1L gene in INT cells following C. parvum infection or in cells following Full-Cdg2_FLc_0220 transfection as measured by using real-time PCR. INT cells following C. parvum infection and cells after transfection of the Full-Cdg2_FLc_0220 for 48 h, followed by real-time PCR analysis of DZLP1L. (D) Downregulation of the DZLP1L gene at the mRNA and protein levels in cells following C. parvum infection or Full-Cdg2_FLc_0220 transfection was further validated in HCT-8 cells. HCT-8 cells following C. parvum infection and cells after transfection of the Full-Cdg2_FLc_0220 for 48 h were collected, followed by real-time PCR and Western blot for DZLP1L. Data represent three independent experiments. *P<0.05 and ** P<0.01, ANOVA versus non-infected or empty vector controls.