Table 1.
Species | Formula | Molecular mass (Da) | Rt (GC-MS) (min)a | M* GC-MS peak (Da)b | Abundance in nmol (% deposited purine) |
---|---|---|---|---|---|
| |||||
Purine | C5H4N4 | 120 | 9.31 | 192 | – |
Hypoxanthine | C5H4N4O | 136 | 12.29 | 280 | 120 (5.7%) |
Xanthine | C5H4N4O2 | 152 | 14.43 | 368 | 1.5 (0.07%)* |
Adenine | C5H5N5 | 135 | 12.71 | 279 | 31 (1.5%) |
2-Aminopurine | C5H5N5 | 135 | 13.20 | 279 | 42 (2.0%) |
2,6-Diaminopurine | C5H6N6 | 150 | 15.46 | 366 | 0.11 (0.0052%) |
Isoguanine | C5H5N5O | 151 | 14.82 | 367 | 0.58 (0.028%) |
Guanine | C5H5N5O | 151 | 15.21 | 367 | 0.25 (0.012%) |
The abundance of xanthine could not be determined accurately because it eluted between two more abundant compounds that may be interfering with the integration. This value should be thus taken as an upper limit.