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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Cell. 2018 Feb 8;172(4):731–743.e12. doi: 10.1016/j.cell.2018.01.007

Figure 6. Loss of TBK1 increases TNFα-induced NFκB activation.

Figure 6

(A, B) IB analysis of eWAT (A) and iWAT (B) from HFD-fed Flox and ATKO mice. (C) IB analysis of differentiated primary preadipocytes treated with TNFα (10nM) for indicated time. (D) Ccl2 expression in differentiated primary preadipocytes treated with TNFα (5nM) for 16hrs. N=3. (E) IB analysis of WT, NKO (NIK KO), TKO (TBK1 KO) or DKO (NIK/TBK1 double KO) MEFs treated with TNFα (10nM) for 45min. (F) Ccl2 expression in WT, NKO, TKO or DKO MEFs treated with TNFα (5nM) for 16hrs. N=3. Data are represented as mean±s.d. *, p<0.05. (G) Proposed model of TBK1-mediated attenuation of NFκB activation. See also Figure S6.