Reagents	Source	Identifier
Chemicals and Recombinant Peptides
Recombination Mouse TNFα	Thermo Scientific	PMC3014
Amlexanox	Abcam	ab142825
AICAR	Cell Signaling Technology	9944
Compound C	Calbiochem	171260
SBI-0206965	Sigma-Aldrich	SML1540
5Z-7-Oxozeaenol	Sigma-Aldrich	O9890
MG132	Sigma-Aldrich	M8699
Lipopolysaccharide	Sigma-Aldrich	L4391
Protease inhibitor tablet	Sigma-Aldrich	11873580001
2-[C14]-deoxyglucose	PerkinElmer	NEC720A250UC
ULK1 active kinase	Signal Chem	U01-11G-10
Antibodies
TBK1 antibody	Cell Signaling Technology	3013
Phospho-TBK1 Ser172 antibody	Cell Signaling Technology	5483
AMPKα antibody	Cell Signaling Technology	2532
Phospho-AMPKα Thr172 antibody	Cell Signaling Technology	2535
p100/p52 antibody	Cell Signaling Technology	4882
Phosphor-IKKα/β antibody	Cell Signaling Technology	2078
p105 antibody	Cell Signaling Technology	4717
Phosphor-p105 Ser933 antibody	Cell Signaling Technology	4806
ULK1 antibody	Cell Signaling Technology	8054
NIK antibody	Cell Signaling Technology	4994
ACC antibody	Cell Signaling Technology	3662
Phospho-ACC Ser79 antibody	Cell Signaling Technology	3661
MAPK (Erk1/2) antibody	Cell Signaling Technology	9102
β-tubulin antibody	Cell Signaling Technology	2146
Myc antibody	Santa Cruz Biotechnology	sc-40
Flag antibody	Sigma-Aldrich	F1804
Oligonucleotides
Primers for Q-PCR, see Table S2	This paper	N/A
Critical Commercial Assays
Ultra-sensitive mouse insulin ELISA kit	Crystal Chem	90080
Triglycerides liquid reagents	Pointe Scientific	T7532
NEFA-HR(2) assay	Wako	434-91795 434-91995
Cyclic AMP XP assay kit	Cell Signaling Technology	4339
Free glycerol determination kit	Sigma-Aldrich	F6428
Extracellular oxygen consumption assay	Abcam	ab197243
NAD/NADH assay kit	Abcam	ab65348
AMP-Glo assay	Promega	V6930
ADP-Glo assay	Promega	V5011
ENLITEN(R) ATP assay	Promega	PAFF2000
Recombinant DNA
pcDNA-Flag-TBK1	Gift from Kate Fitzgerald	N/A
pcDNA-HA-AMPKα1	Gift from Ken Inoki	N/A
pcDNA-HA-AMPKα2	Gift from Ken Inoki	N/A
pcDNA-Flag-AMPKβ1	Gift from Ken Inoki	N/A
pcDNA-Myc-AMPKγ1	Gift from Ken Inoki	N/A
pLentiCRISPR-v2-NIK-gRNA	Gift from Michael Karin	N/A
Experimental Models: Cell Lines
TBK1 WT/KO MEFs	Gift from Kate Fitzgerald	N/A
AMPKα1/α2 WT/KO MEFs	Gift from Ken Inoki	N/A
Immortalized ULK1 WT MEFs	Sigma-Aldrich	14050804-1VL
Immortalized ULK1 KO MEFs	Sigma-Aldrich	14050807-1VL
Experimental Models: Organisms/Strains
Mouse: Tbk1fl/fl	Gift from Kate Fitzgerald	N/A
Mouse: Prkaa1fl/fl, Prkaa2fl/fl	Gift from Ken Inoki	N/A
Mouse: Ulk1fl/fl	Jackson Laboratory	017976
Mouse: Adiponectin-cre	Jackson Laboratory	028020
Mouse: C57BL/6J	Jackson Laboratory	000664
Software
ImageJ	NIH	N/A
GraphPad Prism 6	GraphPad Software	N/A
Other
High-Fat Diet (45% kcal% fat)	Research Diet, Inc.	D12451