Figure 2.

Relative production of proinflammatory cytokines in peritoneal cells of mice stressed with cold water. The levels of cytokines IL-1β (a), TNF-α (b) and IL-6 (c) were measured by using ELISA. Nitric oxide concentrations determined using the Greiss reagent (d), IL-10 (e), TNF-α (f), IL-2 (g) and INF-γ (h). SI, stressed/infected; NSI, non-stressed/infected; NSNI, non-stressed/non-infected mice; APC, antigen-presenting cell; CHL, Chlamydia trachomatis antigen (irradiated); NE, norepinephrine. Each point value represents the means ± standard deviation of 2 or 3 wells (n = 5 or 6 mice for each group of experiments). Statistical differences between treatments were evaluated by Student's t test. Asterisk denotes significant statistical differences between the experimental groups at the level of (P ≤ 0.05).