Table 1.
Primer name | Sequence (5′–3′) |
---|---|
AlsS-f | CGGGGTACCATGTTGACTAAGGCTACTAAGGAACAAA |
AlsS-r | TCCCCGCGGTTACAAAGCTTTAGTCTTCATCAAC |
AlsD-f | CGGAATTCAAAATGAAGAGAGAGTCCAACATCCAAG |
AlsD-r | CGGGGTACCTTATTCTGGTGATCCCTCGGTTGTT |
BDH1-f | CGGAATTCAAAATGAGAGCTTTGGCATATTTCAAGA |
BDH1-r | CGGGGTACCTTACTTCATTTCACCGTGATTGTTA |
udhA-f | CGGGGTACCAAAATGCCACATTCCTACGATTACGATG |
udhA-r | TGCTCTAGATTAAAACAGGCGGTTTAAACCGTTT |
HIS4-f | GAAGATCTATGACATTTCCCTTGCTACCTGC |
HIS4-r | CGGGATCCTTAAATAAGTCCCAGTTTCTCCATACG |
Restriction sites are underlined