Table 3.
Comparison of metabolite production of three P. pastoris strains
| Strains | X33-SD | X33-SDB | X33-SDU |
|---|---|---|---|
| OD600 | 27.15 ± 1α | 29.68 ± 2.08α | 27.23 ± 0.62α |
| 2,3-BD (g/L) | 12.2 ± 0.84α | 8.04 ± 0.95β | 7.44 ± 0.19β |
| Acetoin (g/L) | 1.84 ± 0.76α | 1.03 ± 0.09α | 0.54 ± 0.07β |
| Glycerol (g/L) | 6.42 ± 0.62α | 3.14 ± 0.28β | 2.94 ± 0.23β |
| Ethanol (g/L) | 2.28 ± 0.36α | 6.43 ± 0.08β | 4.96 ± 0.07γ |
Cultivation of three strains was carried out in 500-mL shake flasks containing 100 mL YP media with 40 g/L glucose. Cultivation last for 36 h. Data indicates average values of triplicate experiments with standard deviation. Values with different Greek symbols between columns were significantly different (P < 0.05). ND indicates not detected