Figure 5.

Recruitment of KIF21A to FAs by KANK1 depends on the site-I and site-II interactions. A and B, COS7 cells transiently transfected with GFP-KIF21A (A) and GFP-KANK1 (B). Anti-vinculin antibody (red) is used to stain FA. Scale bar, 10 μm. C, fluorescence-intensity profiles represent protein localization in the area marked by the white arrow in B. D, cotransfection of GFP-KIF21A and Ruby-KANK1 in COS7 cells showing that KIF21A is recruited by KANK1 to the FA border. However, the recruitment is compromised by the two KIF21A mutants, R1156E and L1165Q, disrupting the site-I and site II interactions, respectively. E, results in D were further confirmed by line profile analysis.