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Surface electrostatic potential of the ANKRDs in KANK2/3/4. A, electrostatic potential mapped onto the molecular surface of KANK2_ANKRD (Protein Data Bank code 4HBD) and homology models of the ANKRDs of KANK3 and KANK4. Regions corresponding to site-I are indicated by dashed boxes. The surface analysis clearly shows that site-I is relatively neutral in KANK4. B and C, ITC (B) and gel filtration (C) analysis demonstrate that KANK4_ANKRD cannot bind to KIF21A_H.
