Figure 6.

MSCs internalize amyloid particles of different sizes. A–D, representative images showing internalization of polystyrene beads and AβOs by MSCs and their localization in acidic, LysoTracker-positive compartments. Images were acquired on a Zeiss LSM510 META confocal microscope using a Plan-Neofluar ×63/1.25 numerical aperture oil objective; scale bar, 10 μm. E, representative image (acquired in the LSM510 META confocal microscope using a Plan Apochromat ×100/1.46 numerical aperture oil M27 objective) showing that fibrillar (thioflavin S–positive) Aβ aggregates are observed within lysosomes (LAMP-1–immunolabeling) after 3 h of exposure of MSCs to Aβ fibrils instead of AβOs. Scale bar, 20 μm.