Figure 4.

Inhibition of HDAC6 by ACY-1215 increases ERK1 and ERK2 acetylation. A, ACY-1215 increases ERK1 acetylation in a dose-dependent manner. The experiments were performed the same as described in Fig. 3A, except that ACY-1215 was used for the treatment instead of TSA. B, ACY-1215 increases ERK2 acetylation in a dose-dependent manner. The experiments were performed the same as described in A, except that GST-ERK2 was used for transfection instead of GST-ERK1. A, four independent experiments were performed. B, six independent experiments were performed. Student's t tests were performed with *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.D. IB, immunoblot.