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. 2017 Dec 27;293(6):2159–2171. doi: 10.1074/jbc.M117.808881

Figure 4.

Figure 4.

cml38 mutants are insensitive to the inhibitory activity of AtRALF1 on root growth, and sensitivity is restored by exogenous treatment with CML38 or by introgression of the PROCML38:CML38-GFP gene. A, Arabidopsis seedlings (2 days old) were treated with AtRALF1 for 48 h. Values are the means ± S.D. (error bars) of at least 25 seedlings. ***, p < 0.001 (Student's t test). ns, not significant. B, 2-day-old seedlings were transferred to medium containing 5 μm of AtRALF1, and the root length was measured after 6 days of treatment. Data represent mean values ± S.D. (n = 15). Student's t tests were used to compare treated with AtRALF1 and water control. ***, p < 0.001. C, Arabidopsis seedlings (2 days old) were treated with AtRALF1 (R1), CML38, or CML39 proteins for 48 h. Values are means ± S.D. (error bars) of 35 seedlings. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Student's t test). D, introgression of the PROCML38:CML38-GFP gene into the cml38-1 mutant restores the root growth inhibition caused by AtRALF1. Two-day-old parental lines cml38-1 and PROCML38:CML38-GFP or F3 progeny PROCML38:CML38-GFP/cml38-1 were treated with water or AtRALF1 for 3 days. Values are the means ± S.D. of at least 30 seedlings. ***, p < 0.01 (Student's t test). E, Arabidopsis seedlings (2 days old) were treated with water, AtRALF1 (R1), 12 μg of anti-CML38 (anti-CML) antibody, 12 μg of preimmune serum, or combinations of the peptide with anti-CML or preimmune serum for 48 h. Values are means ± S.D. of 30 seedlings. ***, p < 0.001 (Student's t test).