Figure 2.

Reduction of β1,4GalTV expression inhibits gliomagenesis and transdifferentiation of glioma stem-like cells into the endothelium in vivo. A–C, Kaplan–Meier survival curves of nude mice injected with T698968 (A and B) or T109002 (C) cells expressing control or β1,4GalTV shRNA. D, confocal immunofluorescence analysis shows co-localization of the human endothelial cell marker CD31 and the tumor cell marker GFP in tumor xenografts formed by T698968 cells expressing GFP and control or β1,4GalTV shRNA. Scale bars = 10 μm. E, the relative vascular density derived from glioma stem-like cells was quantified. Results are expressed as mean ± S.E. (n = 3; *, p < 0.05; ***, p < 0.001). F, Western blot analysis of the endothelial cell marker CD31 in xenografts formed by T698968 cells expressing control or β1,4GalTV shRNA. GAPDH protein expression served as a loading control.