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. 2017 Dec 5;121(2):229–240. doi: 10.1093/aob/mcx149

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© The Authors 2017. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — (A) SNP characterization of Ae. speltoides introgressions in five consecutive generations, i.e. BC₁, BC₂, BC₃, BC₄ and BC₅. (B) Genomic in situ hybridization image of the BC₅ genotype (aneuploid 41-chromosome plant). In the SNP characterization red colour is used to represent the presence of an Ae. speltoides introgression and blue colour represents wheat. It should be noted that these diagrams cannot be used to assess which wheat chromosomes the Ae. speltoides segments have recombined with. The GISH image shows a metaphase spread of BC₅-5A probed with labelled genomic DNA of Ae. speltoides. Arrows show Ae. speltoides introgressions (green).