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. 2017 Dec 5;121(2):229–240. doi: 10.1093/aob/mcx149

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© The Authors 2017. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Genomic in situ hybridization showing recombination between Ae. speltoides and the B and D genomes of wheat. (A) GISH analysis of a 41-chromosome BC₃F₁ individual. Metaphase spread with labelled genomic Ae. speltoides DNA as probe showing Ae. speltoides (green) introgressions (white arrows). (B) Same metaphase spread as (A) with three-colour GISH showing Ae. speltoides recombined with the D genome (red arrows). Enlarged inset shows one of the recombined chromosomes. This individual carries 14 A chromosomes (green), 13 B chromosomes (purple), 12 D chromosomes (red) and two wheat/Ae. speltoides 2S recombinant chromosomes (the segment of the D genome is red and marked with the red arrow). (C) GISH analysis of a 42-chromosome BC₅ individual. Metaphase spread with labelled genomic Ae. speltoides (green) DNA as probe showing one complete Ae. speltoides chromosome (blue arrow) from linkage group 2S and one wheat/Ae. speltoides recombined chromosome (white arrow) from Ae. speltoides linkage group 5S. (D) Same metaphase spread as (C) with three-colour GISH showing Ae. speltoides recombined with the B genome (yellow arrow). Enlarged insert shows the recombined chromosome. This individual carries 14 A chromosomes (green), 12 B chromosomes (purple), 14 D chromosomes (red), one complete Ae. speltoides chromosome (purple) and one wheat/Ae. speltoides recombinant chromosome (purple).