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A) CGI-58 exerts acyl-CoA dependent lysophosphatidic acid acyl transferase activity (LPAAT), where lysophosphatidic acid (LPA) and a Coenzyme A (CoA) activated acyl chain are esterified to form phosphatidic acid (PA). B) Catalytic His residues, which are properly positioned by an Asp and then activate the hydroxyl group for nucleophilic attack on acyl-CoA, are often found to be essential in the catalytic reaction of acyl- and acetyl-transferases.
