Sequence alignment of CGI-58 homologs from human (hCGI-58), pig (pCGI-58), mouse (mCGI-58), chicken (cCGI-58), Arabidopsis thaliana (At4g24160), rice (osCGI-58), and Saccharomyces cerevisiae (Ict1p). Conserved regions harboring the G-X-S/N-X-G motif, HX4D motif, and the proposed catalytic Asp in a long loop region are highlighted in yellow. The cap region is highlighted in gray. The sequence alignment was obtained with ClustalW and manual finishing.